For our studies on wound healing, neutrophil kinematics, and the overall immune response to skin infections, we employ the Xenogen IVIS200 for non-invasive, real-time, whole-animal imaging of CL57BL/6 mice that produce neutrophils expressing EGFP. We employ bioluminescent strains of Staphylococcus aureus and Pseudomonas aeruginosa to study and engineer the innate immune response to invading pathogens, in particular, the process of granulopoiesis that hematopoietic stem cells undergo at the site of infection.